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Abnova sheep anti-vsx2 antibody
Sheep Anti Vsx2 Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sheep+anti-vsx2+antibody/pmc06445593-61-70-77?v=Abnova
Average 90 stars, based on 1 article reviews

sheep anti-vsx2 antibody - by Bioz Stars, 2026-07

90/100 stars

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( A ) A scheme of the procedure. ( B ) Diagrams of developing CONCEPT organoids showing concentric zones of the anterior ectodermal progenitors. A summary of , and , – . ( C ) Morphology of cysts at day 2 showing the epithelial structure indicated by apical localization of the reporter TJ::GFP at the lumen. ( D ) Morphology of CONCEPT organoids at day 26. ( E–G ) Expression of telencephalon (Tel) marker Foxg1, neuroretinal (NR) markers Vsx2 and Pax6 in mouse eyes at E10-10.5. Rostral optic stalk (OS) connected the telencephalic vesicle to the optic cup. ( H–O ) FOXG1+ telencephalic progenitors, VSX2+ and/or PAX6+ retinal progenitors formed concentric zones in CONCEPT organoids. N>5 experiments. ( P–W ) In CONCEPT organoids, morphogens FGF8, BMP4, and BMP7 mRNA expression started at early stages and subsequently formed circular gradients. N>5 experiments. Scale bars, 100 µm ( C, E, M, O, P, S, T ), 200 µm ( I, K ), 500 µm ( Q, U ), 1 mm ( D, H, J, L, N, R, V, W ).

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: ( A ) A scheme of the procedure. ( B ) Diagrams of developing CONCEPT organoids showing concentric zones of the anterior ectodermal progenitors. A summary of , and , – . ( C ) Morphology of cysts at day 2 showing the epithelial structure indicated by apical localization of the reporter TJ::GFP at the lumen. ( D ) Morphology of CONCEPT organoids at day 26. ( E–G ) Expression of telencephalon (Tel) marker Foxg1, neuroretinal (NR) markers Vsx2 and Pax6 in mouse eyes at E10-10.5. Rostral optic stalk (OS) connected the telencephalic vesicle to the optic cup. ( H–O ) FOXG1+ telencephalic progenitors, VSX2+ and/or PAX6+ retinal progenitors formed concentric zones in CONCEPT organoids. N>5 experiments. ( P–W ) In CONCEPT organoids, morphogens FGF8, BMP4, and BMP7 mRNA expression started at early stages and subsequently formed circular gradients. N>5 experiments. Scale bars, 100 µm ( C, E, M, O, P, S, T ), 200 µm ( I, K ), 500 µm ( Q, U ), 1 mm ( D, H, J, L, N, R, V, W ).

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Expressing, Marker

( A–E ) Images of VSX2 and PAX6 expression ( A ), PAX6 ( B ), VSX2 ( C ), DAPI ( D ), and an image at a higher magnification ( E ) are shown. Scale bars, 1 mm ( A ), 200 µm ( E ).

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: ( A–E ) Images of VSX2 and PAX6 expression ( A ), PAX6 ( B ), VSX2 ( C ), DAPI ( D ), and an image at a higher magnification ( E ) are shown. Scale bars, 1 mm ( A ), 200 µm ( E ).

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Expressing

( A–E ) Images of VSX2 and PAX6 expression ( A ), PAX6 ( B ), VSX2 ( C ), DAPI ( D ), and an image at a higher magnification ( E ) are shown. Scale bars, 1 mm ( A ), 100 µm ( E ).

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: ( A–E ) Images of VSX2 and PAX6 expression ( A ), PAX6 ( B ), VSX2 ( C ), DAPI ( D ), and an image at a higher magnification ( E ) are shown. Scale bars, 1 mm ( A ), 100 µm ( E ).

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Expressing

N>5 experiments. ( A–D ) POU4F2+ RGCs grew TUBB3+ axons toward and then along a path with a circular or a portion of circular shape. ( E, F ) In mice, Pax2 was expressed in central regions of the retina and optic stalk at E10.5 ( E ) and in the optic disc and optic stalk at E13.5 ( F ). Tubb3+ axons from the initial RGCs grew toward the optic disc, exited the eye, and navigated within the optic stalk ( G ). ( H–L ) In CONCEPT organoids at day 26, TUBB3+ RGC axons grew toward and then along a path defined by an adjacent PAX2+ VSX2+ cell population (arrowhead in H, brackets in I, J ); the PAX2+ VSX2- cell population set up an inner boundary of RGC axon growth. ( L ) A diagram summarizing RGC axon growth, PAX2+ VSX2+ optic disc (OD), and PAX2+ VSX2- optic stalk (OS) in CONCEPT organoids. The area labeled by the asterisk may appear as false signals in a low-resolution printout but it is clearly a background in digital display. ( M ) A count of CONCEPT organoids showing directional retinal ganglion cell axons. Scale bars, 50 µm ( E, F, G ), 100 µm ( B ), 200 µm ( A, H, I ).

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: N>5 experiments. ( A–D ) POU4F2+ RGCs grew TUBB3+ axons toward and then along a path with a circular or a portion of circular shape. ( E, F ) In mice, Pax2 was expressed in central regions of the retina and optic stalk at E10.5 ( E ) and in the optic disc and optic stalk at E13.5 ( F ). Tubb3+ axons from the initial RGCs grew toward the optic disc, exited the eye, and navigated within the optic stalk ( G ). ( H–L ) In CONCEPT organoids at day 26, TUBB3+ RGC axons grew toward and then along a path defined by an adjacent PAX2+ VSX2+ cell population (arrowhead in H, brackets in I, J ); the PAX2+ VSX2- cell population set up an inner boundary of RGC axon growth. ( L ) A diagram summarizing RGC axon growth, PAX2+ VSX2+ optic disc (OD), and PAX2+ VSX2- optic stalk (OS) in CONCEPT organoids. The area labeled by the asterisk may appear as false signals in a low-resolution printout but it is clearly a background in digital display. ( M ) A count of CONCEPT organoids showing directional retinal ganglion cell axons. Scale bars, 50 µm ( E, F, G ), 100 µm ( B ), 200 µm ( A, H, I ).

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Labeling

CONCEPT organoids at day 24 were used for profiling. ( A ) Identification of 14 cell clusters. ( B ) Cell cycle phases revealed by cell cycle scores. ( C ) FOXG1 expression marked telencephalic cells. ( D, E ) The expression of PAX6 and/or VSX2 marked retinal cells. ( F ) PAX2+ cells were found in two major cell populations: PAX2+ VSX2+ cells were assigned as the optic disc (OD), whereas PAX2+ VSX2- FOXG1+ cells were assigned as the optic stalk (OS). ( G–L ) The expression of major DEGs in cluster 2, the major cell population that mimics the optic disc. ( M–P ) The expression of major gene markers for PAX2+ VSX2- optic stalk cells. ( Q–T ) Identification of CNTN2 as a specific marker for early human RGCs. A large portion of cluster 11 differentially expressed neurogenic retinal progenitor marker ATOH7 and RGC markers POU4F2 and SNCG . The expression of CNTN2 and POU4F2 largely overlapped. ( U–X ) Two small portions of cluster 11 differentially expressed early photoreceptor cell markers ( OTX2 and CRX , U, V ) and amacrine/horizontal cell markers ( TFAP2C and PTF1A, W, X ), respectively.

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: CONCEPT organoids at day 24 were used for profiling. ( A ) Identification of 14 cell clusters. ( B ) Cell cycle phases revealed by cell cycle scores. ( C ) FOXG1 expression marked telencephalic cells. ( D, E ) The expression of PAX6 and/or VSX2 marked retinal cells. ( F ) PAX2+ cells were found in two major cell populations: PAX2+ VSX2+ cells were assigned as the optic disc (OD), whereas PAX2+ VSX2- FOXG1+ cells were assigned as the optic stalk (OS). ( G–L ) The expression of major DEGs in cluster 2, the major cell population that mimics the optic disc. ( M–P ) The expression of major gene markers for PAX2+ VSX2- optic stalk cells. ( Q–T ) Identification of CNTN2 as a specific marker for early human RGCs. A large portion of cluster 11 differentially expressed neurogenic retinal progenitor marker ATOH7 and RGC markers POU4F2 and SNCG . The expression of CNTN2 and POU4F2 largely overlapped. ( U–X ) Two small portions of cluster 11 differentially expressed early photoreceptor cell markers ( OTX2 and CRX , U, V ) and amacrine/horizontal cell markers ( TFAP2C and PTF1A, W, X ), respectively.

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Expressing, Marker

Related also to . The dataset deposited by Gabriel et al. was used for plotting. ( A–D ) Cell clustering was reproduced ( A, C ). In Gabriel et al.’s organoids on days 30 and 60, PAX2+ cells were extremely rare ( B, D ). Instead of forming cell clusters, a few PAX2+ cells were scattered across datasets. ( E–G ) In Gabriel et al.’s organoids on day 30, FOXG1+ telencephalic progenitors, SIX3+ retinal progenitors, and ATOH7+ neurogenic retinal progenitors were extremely rare; a few positive cells were scattered across the dataset. VSX2 was not found in the dataset of Gabriel et al.’s organoids on day 30; it was filtered out probably due to its extremely low expression.

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: Related also to . The dataset deposited by Gabriel et al. was used for plotting. ( A–D ) Cell clustering was reproduced ( A, C ). In Gabriel et al.’s organoids on days 30 and 60, PAX2+ cells were extremely rare ( B, D ). Instead of forming cell clusters, a few PAX2+ cells were scattered across datasets. ( E–G ) In Gabriel et al.’s organoids on day 30, FOXG1+ telencephalic progenitors, SIX3+ retinal progenitors, and ATOH7+ neurogenic retinal progenitors were extremely rare; a few positive cells were scattered across the dataset. VSX2 was not found in the dataset of Gabriel et al.’s organoids on day 30; it was filtered out probably due to its extremely low expression.

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Expressing

( A ) Cell clustering of human fetal retinas HGW9 (GSE138002) identified 21 clusters. ( B–F ) Cluster 18 differentially expressed PAX2, COL9A3, CYP1B1, SEMA5A, and FGF9 , which were top DEGs of cluster 2 in CONCEPT organoids. ( G ) VSX2 expression marked retinal progenitor cells. ( H–M ) Identification of neurogenic retinal progenitor cells ( H ), early photoreceptor cells ( I ), amacrine/horizontal cells ( J, K ), and early RGCs ( L, M ). POU4F2 and CNTN2 were largely co-expressed in early RGCs, consistent with their expression profiles in CONCEPT organoids. ( N–R ) When cells in cluster 18 and retinal progenitors from HGW9 were combined with cells in clusters 2, 4, 5, 7 from CONCEPT organoids (CR24) for Seurat anchor-based clustering, cells in cluster 18 from HGW9 (H18) were grouped with cluster 2 from CONCEPT organoids (C2, assigned optic disc; N ), and these cells expressed both PAX2 and VSX2 (arrowheads in N-R). A small portion of H18 cells were grouped with C4 cells (assigned optic stalk; N ), and these cells expressed PAX2 but not VSX2 (arrows in N-R).

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet: ( A ) Cell clustering of human fetal retinas HGW9 (GSE138002) identified 21 clusters. ( B–F ) Cluster 18 differentially expressed PAX2, COL9A3, CYP1B1, SEMA5A, and FGF9 , which were top DEGs of cluster 2 in CONCEPT organoids. ( G ) VSX2 expression marked retinal progenitor cells. ( H–M ) Identification of neurogenic retinal progenitor cells ( H ), early photoreceptor cells ( I ), amacrine/horizontal cells ( J, K ), and early RGCs ( L, M ). POU4F2 and CNTN2 were largely co-expressed in early RGCs, consistent with their expression profiles in CONCEPT organoids. ( N–R ) When cells in cluster 18 and retinal progenitors from HGW9 were combined with cells in clusters 2, 4, 5, 7 from CONCEPT organoids (CR24) for Seurat anchor-based clustering, cells in cluster 18 from HGW9 (H18) were grouped with cluster 2 from CONCEPT organoids (C2, assigned optic disc; N ), and these cells expressed both PAX2 and VSX2 (arrowheads in N-R). A small portion of H18 cells were grouped with C4 cells (assigned optic stalk; N ), and these cells expressed PAX2 but not VSX2 (arrows in N-R).

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Expressing

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Western Blot, Labeling, Sequencing, Software

Journal: eLife

Article Title: Self-formation of concentric zones of telencephalic and ocular tissues and directional retinal ganglion cell axons

doi: 10.7554/eLife.87306

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9016 , IF (1:500).

Techniques: Western Blot, Labeling, Sequencing, Software

Journal: eLife

Article Title: m 6 A epitranscriptomic modification regulates neural progenitor-to-glial cell transition in the retina

doi: 10.7554/eLife.79994

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-VSX2 (sheep polyclonal) , Millipore , Cat# AB9014; RRID :AB_262173 , 1:500.

Techniques: Recombinant, Plasmid Preparation, Over Expression, shRNA, Expressing, Sequencing

D18 OVs from VSX2WT (A–C) or VSX2R200Q (D–F) hiPSCs were immunostained for VSX2 (red) and MITF (green) (A, D), MITF (red) and WLS (green) (B, E), or VSX2 (red) and WLS (green) (C, F). D35 and d50 VSX2WT hiPSC-OVs (G–I) were examined for VSX2 and MITF (G,I) or VSX2 and WLS (H) expression and compared to d35 (J,K) and d50 (L) VSX2R200Q hiPSC-OVs. Identical images with DAPI-labeled nuclei are shown in Fig. S2. Scale bars = 50 μm.

Journal: Stem cells (Dayton, Ohio)

Article Title: Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

doi: 10.1002/stem.2414

Figure Lengend Snippet: D18 OVs from VSX2WT (A–C) or VSX2R200Q (D–F) hiPSCs were immunostained for VSX2 (red) and MITF (green) (A, D), MITF (red) and WLS (green) (B, E), or VSX2 (red) and WLS (green) (C, F). D35 and d50 VSX2WT hiPSC-OVs (G–I) were examined for VSX2 and MITF (G,I) or VSX2 and WLS (H) expression and compared to d35 (J,K) and d50 (L) VSX2R200Q hiPSC-OVs. Identical images with DAPI-labeled nuclei are shown in Fig. S2. Scale bars = 50 μm.

Article Snippet: 10% volume was reserved for input and the remainder was incubated with 2 μg sheep anti-VSX2 antibody (Exalpha) overnight at 4°C with rocking.

Techniques: Expressing, Labeling

Confirmation of the VSX2 consensus binding motif in VSX2WT hiPSC-OV ChIP-seq target sequences (A). Distribution of high confidence VSX2 DNA binding targets in VSX2WT hiPSC-OVs as categorized by genomic location (B) or RNA species (C). List of GO terms with greater than 3-fold enrichment generated via DAVID functional analysis of high confidence ChIP-seq peaks (D). Peak localization and genomic coverage maps (± 200 bp toward the 5' or 3' end) for WLS, AXIN2, and WNT1 (red and blue lines represent forward and reverse DNA strand reads, respectively). The top panel designates ChIP coverage and the lower panel shows the input control for each gene (E). VSX2 ChIP-PCR from VSX2WT or VSX2R200Q d30 hiPSC-OVs confirming direct binding of VSX2WT, but not VSX2R200Q, to targets identified proximal to the WNT signaling pathway genes WLS, AXIN2, and WNT1 (F). Regions in the MITF-H promoter previously shown by ChIP-PCR to be bound (+) or not bound (−) by VSX220 served as positive and negative controls, respectively (amplified from the same chromatin preparation presented in panel F) (G). See also Fig. S1.

Journal: Stem cells (Dayton, Ohio)

Article Title: Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

doi: 10.1002/stem.2414

Figure Lengend Snippet: Confirmation of the VSX2 consensus binding motif in VSX2WT hiPSC-OV ChIP-seq target sequences (A). Distribution of high confidence VSX2 DNA binding targets in VSX2WT hiPSC-OVs as categorized by genomic location (B) or RNA species (C). List of GO terms with greater than 3-fold enrichment generated via DAVID functional analysis of high confidence ChIP-seq peaks (D). Peak localization and genomic coverage maps (± 200 bp toward the 5' or 3' end) for WLS, AXIN2, and WNT1 (red and blue lines represent forward and reverse DNA strand reads, respectively). The top panel designates ChIP coverage and the lower panel shows the input control for each gene (E). VSX2 ChIP-PCR from VSX2WT or VSX2R200Q d30 hiPSC-OVs confirming direct binding of VSX2WT, but not VSX2R200Q, to targets identified proximal to the WNT signaling pathway genes WLS, AXIN2, and WNT1 (F). Regions in the MITF-H promoter previously shown by ChIP-PCR to be bound (+) or not bound (−) by VSX220 served as positive and negative controls, respectively (amplified from the same chromatin preparation presented in panel F) (G). See also Fig. S1.

Article Snippet: 10% volume was reserved for input and the remainder was incubated with 2 μg sheep anti-VSX2 antibody (Exalpha) overnight at 4°C with rocking.

Techniques: Binding Assay, ChIP-sequencing, Generated, Functional Assay, Amplification

List of WNT-related Genes Identified by VSX2 ChIP-SEQ

Journal: Stem cells (Dayton, Ohio)

Article Title: Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

doi: 10.1002/stem.2414

Figure Lengend Snippet: List of WNT-related Genes Identified by VSX2 ChIP-SEQ

Article Snippet: 10% volume was reserved for input and the remainder was incubated with 2 μg sheep anti-VSX2 antibody (Exalpha) overnight at 4°C with rocking.

Techniques:

D14 VSX2WT (A–H) or VSX2R200Q (I–P) hiPSC-OVs were immunolabeled with βCATENIN (green), VSX2 (red), and MITF (blue) primary antibodies. Examples of βCATENIN nuclear localization are designated by white arrows (E–H and M–P). Panels E–H and M–P are cropped magnifications of the outlined areas in panels A–D and I–J, respectively. Also see Fig. S3 for identical images with βCATENIN and DAPI-labeled nuclei. (Q) Graph of percent of βCATENIN nuclear localization in VSX2+ nuclei for WT and R200Q d14 hiPSC-OVs. **p=0.01. Scale bars = 50 μm.

Journal: Stem cells (Dayton, Ohio)

Article Title: Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

doi: 10.1002/stem.2414

Figure Lengend Snippet: D14 VSX2WT (A–H) or VSX2R200Q (I–P) hiPSC-OVs were immunolabeled with βCATENIN (green), VSX2 (red), and MITF (blue) primary antibodies. Examples of βCATENIN nuclear localization are designated by white arrows (E–H and M–P). Panels E–H and M–P are cropped magnifications of the outlined areas in panels A–D and I–J, respectively. Also see Fig. S3 for identical images with βCATENIN and DAPI-labeled nuclei. (Q) Graph of percent of βCATENIN nuclear localization in VSX2+ nuclei for WT and R200Q d14 hiPSC-OVs. **p=0.01. Scale bars = 50 μm.

Article Snippet: 10% volume was reserved for input and the remainder was incubated with 2 μg sheep anti-VSX2 antibody (Exalpha) overnight at 4°C with rocking.

Techniques: Immunolabeling, Labeling

Immunocytochemistry analysis on d18 VSX2WT hiPSC-OVs treated with the WNT agonist CHIR99201 (A–C) or vehicle (D–F) showing VSX2 (red) and MITF (green) coexpression (A–C) or lack thereof (D–F). RT-qPCR analysis of d30 VSX2WT hiPSC-OVs treated from d14–d20 with vehicle or CHIR99201 (G). Immunocytochemistry analysis on d18 VSX2R200Q hiPSC-OVs treated with the WNT inhibitor IWP2 (H–J) or vehicle (K–M) showing lack of coexpression (H–J) or coexpression (K–M) of VSX2 (red) and MITF (green). (N) RT-qPCR analysis of d30 VSX2R200Q hiPSC-OVs treated from d12–d20 with vehicle or inhibitor. Nuclei are shown in blue. *p< 0.01; **p<0.001;***p<0.0001. Scale bars = 50 μm.

Journal: Stem cells (Dayton, Ohio)

Article Title: Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

doi: 10.1002/stem.2414

Figure Lengend Snippet: Immunocytochemistry analysis on d18 VSX2WT hiPSC-OVs treated with the WNT agonist CHIR99201 (A–C) or vehicle (D–F) showing VSX2 (red) and MITF (green) coexpression (A–C) or lack thereof (D–F). RT-qPCR analysis of d30 VSX2WT hiPSC-OVs treated from d14–d20 with vehicle or CHIR99201 (G). Immunocytochemistry analysis on d18 VSX2R200Q hiPSC-OVs treated with the WNT inhibitor IWP2 (H–J) or vehicle (K–M) showing lack of coexpression (H–J) or coexpression (K–M) of VSX2 (red) and MITF (green). (N) RT-qPCR analysis of d30 VSX2R200Q hiPSC-OVs treated from d12–d20 with vehicle or inhibitor. Nuclei are shown in blue. *p< 0.01; **p<0.001;***p<0.0001. Scale bars = 50 μm.

Article Snippet: 10% volume was reserved for input and the remainder was incubated with 2 μg sheep anti-VSX2 antibody (Exalpha) overnight at 4°C with rocking.

Techniques: Immunocytochemistry, Quantitative RT-PCR

VSX2WT acts in multiple ways to maintain NR identity. On a gene expression level, VSX2 antagonizes expression of WNT pathway genes and MITF, resulting in the promotion of NR fate at the expense of RPE in hiPSC-OVs (A). In the absence of functional VSX2 (i.e. VSX2R200Q hiPSC-OVs), expression of WNT pathway genes and MITF are unchecked, leading to RPE production over NR (B).

Journal: Stem cells (Dayton, Ohio)

Article Title: Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

doi: 10.1002/stem.2414

Figure Lengend Snippet: VSX2WT acts in multiple ways to maintain NR identity. On a gene expression level, VSX2 antagonizes expression of WNT pathway genes and MITF, resulting in the promotion of NR fate at the expense of RPE in hiPSC-OVs (A). In the absence of functional VSX2 (i.e. VSX2R200Q hiPSC-OVs), expression of WNT pathway genes and MITF are unchecked, leading to RPE production over NR (B).

Article Snippet: 10% volume was reserved for input and the remainder was incubated with 2 μg sheep anti-VSX2 antibody (Exalpha) overnight at 4°C with rocking.

Techniques: Expressing, Functional Assay

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